Nature

Using virally expressed melanoma cDNA libraries to identify tumor-associated antigens that cure melanoma

Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with ...
Nature

Analysis of 5′ transcript heterogeneity by high-throughput sequencing of cDNA

We isolated mRNA from Blumeria graminis and prepared a cDNA library using a modification of Epicentre's ExactSTART ™ Full-Length cDNA Library Cloning kit. Analysis of the 5′ ends of the cDNA by 454 ...
The Scientist

cDNA Library Construction sans Restriction Enzymes

Complementary DNA (cDNA) libraries are standard tools for gene expression studies. In theory, the library contains full-length DNA copies of every mRNA in the starting sample, in abundances ...
News Medical

Murine Neuronal Cell cDNA Sample Library Prep RNA-Seq

Core laboratories must balance the efficiency of their NGS workflow with the quality of the data that they produce in providing numerous next generation sequencing (NGS) services relating to a wide ...
BioTechniques

RNA sequencing library preparation and construction

What is an RNA sequencing library? In all RNA-seq experiments, a library of cDNA fragments attached to adapters must be synthesized in order for sequencing to be successful. How do you prepare an RNA ...