Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. The name was first used by Raphaël Dubois who …

Jul 23, 2025 · The luciferase enzyme is a class of biological catalysts found across diverse organisms. These enzymes are responsible for bioluminescence, where living beings produce …

Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence. The widespread bioluminescence of such living organisms as fireflies and bacteria is based on …

Jul 16, 2024 · Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light. 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D …

Jul 22, 2025 · A luciferase and a fluorescent protein are each fused to one of the proteins being tested for interaction. If no interaction occurs, the luciferase emits its characteristic light.

Oct 17, 2025 · Bioluminescence is a fascinating natural phenomenon where living organisms emit light through a chemical reaction. This process is orchestrated by a class of enzymes known …

Apr 16, 2024 · Luciferase enzymes regulate bioluminescence in cells and can be found in a variety of organisms. It provides a useful research tool that allows for the study of gene …

So, luciferase uses a very energetic process to create light. It has a cofactor, termed a luciferin, that forms a highly strained complex with oxygen, using an ATP molecule to help set …

Luciferase is an enzyme that catalyzes a light-producing biochemical reaction when it is in the presence of oxygen, ATP, magnesium, and a naturally occurring substrate called luciferin.

Optoanalytical methods based on using genetically encoded bioluminescent enzymes, luciferases, allow one to obtain highly sensitive signals, are non-invasive, and require no …