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Elina on Instagram: "BASIC IMMUNOCYTOCHEMISTRY PROTOCOL Here I stained iPSC-derived cardiac fibroblasts for vimentin (red) and Hoechst (blue). I used chamber slides. 1. Grow cells, treat them with your experimental conditions 2. Fix them in 100% methanol, block in 3% BSA/PBS 3. Add primary antibodies, incubate overnight at 4Co in dark 4. Wash 3x with PBS (5 min/wash) 5. Add secondary fluorescent antibodies. Incubate for 1h at room temperature in the dark. 6. Wash with PBS once 7. Add Hoechst 8.
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